Journal: Computational and Structural Biotechnology Journal

Article Title: Single-molecule optical microscopy of protein dynamics and computational analysis of images to determine cell structure development in differentiating Bacillus subtilis

doi: 10.1016/j.csbj.2020.06.005

Figure Lengend Snippet: . Stoichiometry of SpoIIE in the mother cell. Kernel density estimation (KDE) of the stoichiometry (i.e. number of SpoIIE molecules per detected fluorescent focus) in mother cell with predicted overlap tetramer model (black dotted line).Chi-squared χ 2 and probability of confidence p values indicated. Stage I (green),II i (orange), II ii (red), II iii (yellow) and III (cyan). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Kernel density estimation (KDE) of the stoichiometry (i.e. number of SpoIIE molecules per detected fluorescent focus) in mother cell with predicted overlap tetramer model (black dotted line).Chi-squared χ 2 and probability of confidence p values indicated.

Techniques:

Journal: Computational and Structural Biotechnology Journal

Article Title: Single-molecule optical microscopy of protein dynamics and computational analysis of images to determine cell structure development in differentiating Bacillus subtilis

doi: 10.1016/j.csbj.2020.06.005

Figure Lengend Snippet: . Stoichiometry of SpoIIE in the forespore. (A) Kernel density estimate (KDE) of forespore stoichiometry pooled for all data, and zoom-in (inset), (B) in separate stages with overlap tetramer model (black dotted line). (C) Bar chart of mean foci stoichiometry for foci which have > 20 molecules. Stage II ii statistically higher than II i (p = 0.007) (D) Mean number of foci detected per forespore. (E) Mean foci stoichiometry vs. normalised distance into spore for each stage, (inset) schematic of forespore distance normalization. Stage I (green),II i (orange), II ii (red), II iii (yellow) and III (cyan). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Kernel density estimation (KDE) of the stoichiometry (i.e. number of SpoIIE molecules per detected fluorescent focus) in mother cell with predicted overlap tetramer model (black dotted line).Chi-squared χ 2 and probability of confidence p values indicated.

Techniques: